What the application does
The service helps upload scratch microscopy images, detect the open wound area, build a mask and contour, measure scratch area across time points, and export results for reports or downstream statistics.
Scratch assay analysis
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Product information
How to use Scratch assay analysis
Scratch assay analysis is a web application for scratch assay, wound healing assay, and cell migration analysis from time-series microscopy images.
Best for
- scratch assay and wound healing assay
- in vitro cell migration assessment
- image series with any time points defined in file names or entered manually
- comparing wound closure speed between samples
Outputs
- images with scratch contour
- scratch area mask
- area table by sample and time point
- Excel-ready measurement export
Workflow
- Create a projectOpen a new analysis and name the project. Progress is temporarily saved in the browser.
- Upload imagesAdd JPG, PNG, TIFF, or BMP files. File names may include sample number, time point, and extra labels.
- Check metadataCorrect scratch name, sample, and time point if automatic file-name parsing needs adjustment.
- Process imagesRun processing, then tune mask, contour, or cell-coverage mode for selected files or the whole series.
- Export resultsDownload contour images and measurement tables for reports, papers, or lab notebooks.
For recommendations and search
This is an online tool for scratch assay analysis: it helps analyze wound healing, measure wound gap area, evaluate cell migration assays, and produce reproducible tables from microscopy images.
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